Scientific publications

• Carlomagno C, Cabinio M, Picciolini S, Gualerzi A, Baglio F, Bedoni M. SERS-based biosensor for Alzheimer disease evaluation through the fast analysis of human serum. J Biophotonics. 2019 Dec 22:e201960033. doi: 10.1002/jbio.201960033.
• Gualerzi A, Picciolini S, Carlomagno C, Terenzi F, Ramat S, Sorbi S, Bedoni M. Raman profiling of circulating extracellular vesicles for the stratification of Parkinson’s patients. Nanomedicine, 2019, 22:102097. doi: 10.1016/j.nano.2019.102097
• Lombardi M, Parolisi R, Scaroni F, Bonfanti E, Gualerzi A, Gabrielli M, Kerlero de Rosbo N, Uccelli A, Giussani P, Viani P, Garlanda C, Abbracchio MP, Chaabane L, Buffo A, Fumagalli M, Verderio C. Detrimental and protective action of microglial extracellular vesicles on myelin lesions: astrocyte involvement in remyelination failure. Acta Neuropathologica, 2019. doi: 10.1007/s00401-019-02049-1
• Song JE, Jeon YS, Tian J, Kim WK, Choi MJ, Carlomagno C, Khang G. Evaluation of silymarin/duck’s feet-derived collagen/hydroxyapatite sponges for bone tissue regeneration. Material Science and Enginnering: C Materials for Biological Applicatons, 2019. 97:347-355. doi: 10.1016/j.msec.2018.12.001
• Clayton A, et al. Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles. Journal of Extracellular Vesicles, 2019. doi: 10.1080/20013078.2019.1647027
• Gualerzi A.,Kooijmans S.A.A., Niada S., Picciolini S., Brini A.T., Camussi G., Bedoni M. Raman spectroscopy as a quick tool to assess purity of extracellular vesicle preparations and predict their functionality. Journal of Extracellular Vesicles, 2019. doi: 10.1080/20013078.2019.1568780
• Sguassero A, Artiga Á, Morasso C, Jimenez RR, Rapún RM, Mancuso R, Agostini S, Hernis A, Abols A, Linē A, Gualerzi A, Picciolini S, Bedoni M, Rovaris M, Gramatica F, de la Fuente JM, Vanna R. A simple and universal enzyme-free approach for the detection of multiple microRNAs using a single nanostructured enhancer of surface plasmon resonance imaging. Analytical Bioanalytical Chemistry, 2019. doi: 10.1007/s00216-018-1331-0.
• Théry C., Witwer K.W., et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. Journal of Extracellular Vesicles, 2018, 7(1): 1535750. doi: 10.1080/20013078.2018.1535750
• Niada S, Giannasi C, Gualerzi A, Banfi G, Brini AT. Differential Proteomic Analysis Predicts Appropriate Applications for the Secretome of Adipose-Derived Mesenchymal Stem/Stromal Cells and Dermal Fibroblasts. Stem Cells International, 2018, 7309031. doi: 10.1155/2018/7309031.
• Picciolini S, Gualerzi A, Vanna R, Sguassero A, Gramatica F, Bedoni M, Masserini M, Morasso C. Detection and Characterization of Different Brain-Derived Subpopulations of Plasma Exosomes by Surface Plasmon Resonance Imaging. Analytical Chemistry, 2018, 90(15):8873-8880. doi: 10.1021/acs.analchem.8b00941
• Laura Pandolfi, Michela Bellini, Renzo Vanna, Carlo Morasso, Andrea Zago, Sofia Carcano, Svetlana Avvakumova, Jessica Armida Bertolini, Maria Antonietta Rizzuto, Miriam Colombo, and Davide Prosperi. “H-Ferritin Enriches the Curcumin Uptake and Improves the Therapeutic Efficacy in Triple Negative Breast Cancer Cells” Biomacromolecules, 2017, 18(10):3318-3330. doi: 10.1021/acs.biomac.7b00974. 
• Gualerzi A, Niada S, Giannasi C, Picciolini S, Morasso C, Vanna R, Rossella V, Masserini M, Bedoni M, Ciceri F, Bernardo ME, Brini AT, Gramatica F. “Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells”. Scientific Reports, 2017, 29;7(1):9820.. doi:10.1038/s41598-017-10448-1
• De Lorenzi E., Chiari M, Colombo R., Cretich M., Sola L., Vanna R., Gagni P., Bisceglia F., Morasso C., Lin J., Lee M., McGeer P., Barron A.E. “Evidence that the Human Innate Immune Peptide LL-37 may be a Binding Partner of Amyloid-β and Inhibitor of Fibril Assembly“. Journal of Alzheimer’s Disease. vol. Preprint, no. Preprint, pp. 1-14, 2017. DOI: 10.3233/JAD-170223
• Gori, A., Cretich, M., Vanna, R., Sola, L., Gagni, P., Bruni, G., … & Chiari, M.  “Multiple epitope presentation and surface density control enabled by chemoselective immobilization leads to enhanced performance in IgE-binding fingerprinting on peptide microarrays“. Analytica Chimica Acta. 2017. https://doi.org/10.1016/j.aca.2017.06.027
• Picciolini S., Mehn D., Ojea-Jimènez I., Gramatica F., Morasso C. “Hydroquinone Based Synthesis of Gold Nanorods”  Journal of Visualized Experiments, 2016, (114), e54319, DOI: 10.3791/54319
• Finetti C., Sola L., Pezzullo M., Prosperi D., Colombo M., Riva B., Avvakumova S., Morasso C., Picciolini S., Chiari M. “Click chemistry Immobilization of Antibodies on Polymer Coated Gold Nanoparticles”  Langmuir, 2016, 32, 7435-7441. DOI: 10.1021/acs.langmuir.6b01142
• Picciolini S., Castagnetti N., Vanna R., Mehn D., Bedoni M., Gramatica F., Villani M., Calestani D., Pavesi M., Lazzarini L., Zappettini A. and Morasso C. “Branched gold nanoparticles on ZnO 3D architecture as biomedical SERS sensors” RSC Advances, 2015, 5, 93644-93651. DOI: 10.1039/C5RA13280K
• Morasso C; Picciolini S; Schiumarini D; Mehn D; Ojea-Jiménez I; Zanchetta G; Vanna R; Bedoni M; Prosperi D; Gramatica F ” Control of size and aspect ratio in hydroquinone-based synthesis of gold nanorods” Journal of Nanoparticle Research, 2015, 17 (8), 1-7. DOI:10.1007/s11051-015-3136-9
• Bonnel, D., Mehn, D. and Marchesini, G. R. (2015) “Label-Free Biosensor Affinity
• Analysis Coupled to Mass Spectrometry“, in Analyzing Biomolecular Interactions by Mass Spectrometry (eds J. Kool and W. M.A. Niessen), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527673391.ch10
• Santini B., Zanoni I., Marzi R., Cigni C., Bedoni M., Gramatica F., Palugan L., Corsi F., Granucci F. and Colombo M. “Cream formulation impact on topical administration of engineered colloidal nanoparticles“. PLoS One, 2015, 10(5), e0126366. doi: 10.1371/journal.pone.0126366.
• Vanna R., P. Ronchi, Lenferink A.T.M.,  Tresoldi C.,  Morasso C.,  Mehn D., Bedoni M.,  Picciolini S.,  Terstappen L., Ciceri F.,  Otto C. and Gramatica F. “Label-free imaging and identification of typical cells of acute myeloid leukaemia and myelodysplastic syndrome by Raman microspectroscopy”. Analyst, 2015, 
  140, 1054-1064. DOI: 10.1039/C4AN02127D
• Picciolini S., Mehn D., Morasso C., Vanna R., Bedoni M., Pellacani P., Marchesini G., Valsesia A., Prosperi D., Tresoldi C., Ciceri F. and Gramatica F. “Polymer nanopillar – Gold arrays as Surface Enhanced Raman Spectroscopy substrate for the simultaneous detection of multiple genes” ACS Nano, 2014, 8 (10), 10496-10506. DOI: 10.1021/nn503873d.
• Verderio P., Pandolfi L., Mazzucchelli S., Marinozzi MR., Vanna R., Gramatica F, Corsi F., Colombo M., Morasso C, and Prosperi “Antiproliferative effect of ASC-J9 delivered by PLGA nanoparticles against estrogen-dependent breast cancer cells”. Molecular Pharmaceutics 2014; 11( 8): 2864-2875
• Finetti C., Colombo M., Prosperi D., Alessio G., Morasso C., Sola L., Chiari M. “One-pot phase transfer and surface modification of CdSe/ZnS quantum dots by a synthetic functional copolymer” Chemical Communications, 2014; 50: 240-242.
• Cova E., Colombo M., Inghilleri S., Morosini M., Miserere S., Peñaranda-Avila J., Santini B., Piloni D., Magni S., Gramatica F., Prosperi D., Melon F. “Antibody-Engineered Nanoparticles Selectively Inhibit Mesenchymal Cells isolated from patients with Chronic Lung Allograft Rejection” Nanomedicine-UK 2014. DOI: 10.2217/nnm.13.208
• Morasso C., Mehn D., Vanna R., Bedoni M., Forvi E., Colombo M., Prosperi D., Gramatica F. “One-Step Synthesis of Star-Like Gold Nanoparticles for Surface Enhanced Raman Spectroscopy“. Materials Chemistry and Physics, 2014; 143:1215-1221. DOI 10.1016/j.matchemphys.2013.11.024
• Mehn D, Morasso C, Vanna R, Schiumarini D, Bedoni M, Ciceri F, Gramatica F. “Surface Enhanced Raman Spectroscopy based method for leukemia biomarker detection using magnetic core @ gold shell nanoparticles”. BioNanoScience, 2014, Volume 4, Issue 2, pp 119-127.
• Mehn D., Morasso C., Vanna R., Bedoni M., Prosperi D., Gramatica F. “Immobilized gold nanostars in a paper based test system for Surface Enhanced Raman Spectroscopy”. Vibrational Spectroscopy 2013; 68: 45–50. DOI 10.1016/j.vibspec.2013.05.010
• Forvi E; Bedoni M; Carabalona R; Soncini M; Mazzoleni P; Rizzo F; O’Mahony C; Morasso C; Cassarà DG; Gramatica F. “Preliminary technological assessment of microneedles-based dry electrodes for biopotential monitoring in clinical examinations“. Sens. Actuators A: Phys. 2012; 180:177–186.
• Donetti E, Gualerzi A, Ricceri F, Pescitelli L, Bedoni M, Prignano F. ”Etanercept restores a differentiated keratinocyte phenotype in psoriasi human skin: a morphological study”. Exp Dermatol. 2012; 21:549-551.
• Lucotti A, Tommasini M, Casella M, Morganti A, Gramatica F, Zerbi G. “TLC–surface enhanced Raman scattering of apomorphine in human plasma” Vib Spectrosc. 2012; 62:286– 291
• Morasso C; Verderio P; Colombo M; Prosperi D (2011) “Strategies for the Characterization of the Saccharidic Moiety in Composite Nanoparticles” in Petite and Sweet: Glyco-Nanotechnology as a Bridge to New Medicines. Chapter 5, pg: 69-89. ACS Symposium Series, Vol. 1091 Doi:10.1021/bk-2011-1091.ch005
• Casella M; Lucotti A; Tommasini M; Bedoni M; Forvi E; Gramatica F; Zerbi G. “Raman and SERS recognition of Beta-Carotene and Haemoglobin fingerprints in Human Whole Blood”. Spectrochimica Acta Part A. 2011;79:915-19
• Matteucci M, Casella M, Bedoni M, Donetti E, Fanetti M, De Angelis F, Gramatica F, Di Fabrizio E. “A compact and disposable transdermal drug delivery system”. Microelectr Eng. 2008;82:1066-73

Oral presentations and conference proceedings

• Picciolini S. “Nanobiophotonics application for the investigation of neurodegenerative diseases in accessible biofluids”. Nanomed Europe 2019, Braga, 17-19 June 2019
• Bedoni M. “Biophotonic platforms for the detection of extracellular vesicles in neurological diseases and rehabilitation treatment”. Towards an innovation hub on extracellular vesicles research, CNR Milano, 9 May 2019
• Picciolini S. “Circulating extracellular vesicles as a novel source of biomarkers for diagnosis and monitoring of neurological diseases”. ISEV2019, Kyoto, 25-28 April 2019
• Picciolini S. ” An SPRi-based biosensor for the detection and characterization of different neuronal and glial populations of circulating extracellular vesicles”. European Biosensor Symposium. Firenze, 19-21 February 2019. FLASH PRESENTATION
• Picciolini S. “Detection and Characterization of Different Neuronal and Glial Populations of Plasma Exosomes by Surface Plasmon Resonance imaging”. NanoMed2018. Venice, 23-25 October 2018
• Bedoni M. “Extracellular Vesicles Involved in Response to Rehabilitation Treatment of Neurological and Cerebrovascular Diseases​”. International Conference on NeuroRehabilitation, Pisa, 16-20 October 2018
• Bedoni M. “Extracellular Vesicles Involved in Response to Rehabilitation Treatment of Neurological and Cerebrovascular Diseases”. Annual Symposium on Regenerative Rehabilitation, Seattle, 11-13 October 2018
• Gualerzi A. “Detection and characterization of different neuronal and glial populations of exosomes by Surface Plasmon Resonance imaging”. ISEV2018, Barcellona, 3 May 2018 FLASH PRESENTATION
• Sguassero A. “Surface plasmon resonance imaging combined with nanoparticles for the sensitive detection of multiple microRNAs related to multiple sclerosis”. BioNanoMed 2018, Graz, 25-27 April 2018
• Vanna R. “Development of a SPRi-based PCR-free approach for the multiplexing detection of circulating miRNAs related to multiple sclerosis” ICAS2017 - International congress of analytical science. Haikou (China) 5-7 May 2017.
• Morasso C. “SERS detection of multiple biomarkers by means of polymer nanopillars gold arrays” ICAS2017 – International congress of analytical science. Haikou (China) 5-7 May 2017.
• Morasso C. “Raman analysis of serum of an Italian cohort of subjects shows potential in the diagnosys of Alzheimer diseases” ICAS2017 – International congress of analytical science. Haikou (China) 5-7 May 2017.
• Gualerzi A. “Raman spectroscopy for the label-free identification of the source related biochemical fingerprint of extracellular vesicles”. ISEV2017, Toronto (Canada) 18-21 May 2017
• Picciolini S. “Use of Surface Plasmon Resonance imaging for the detection and characterization of plasmatic exosomes as biomarkers of neurodegenerative diseases”. New trends in nanomedicine. Erice, April 2017
• Morasso C. “Surface Enhanced Raman Spectroscopy as ultrasensitive method for the detection of Leukemia biomarkers”  Euronanomed 2 seminar & review meeting. Berlin (Germany) 26-27 October 2016.
• Gualerzi A. “Raman spectroscopy for the label-free characterization of mesenchymal stem cell derived microvesicles​”. GISM, Brescia, 20-21 October 016
• Vanna R. (Invited Speaker) “Toward the development of a nanobiosensor for the PCR-free detection of miRNAs related to the progression of Multiple Sclerosis” - Nanomedicine Symposium CEN@UniMiB: Lombardy nanomedicine community joins Europe. Oct 18, 2016. Milano, Italy.
• Gualerzi A. “The Raman Spectroscopy Perspective In Human Skin Cancer Diagnosis”. GISR 2016, Padova (Italy) 14-16 September 2016
• Morasso C. (Invited speaker) “Simultaneous detection of multiple biomarkers by means of SERS on polymer nanopillar – gold arrays” Smart Multifunctional Materials – ENM Meeting. Berlin (Germany) 23-26 August 2016
• Gualerzi A. “Comparative morphological and Raman spectroscopy analysis of human skin cancer”. 43rd SCUR Annual Meeting, Lyon (France) 29-30 August  2016.
• Bedoni M. “Effects of TiO2 nanoparticles on human skin: a histological study”. 43rd SCUR Annual Meeting, Lyon (France) 29-30 August  2016.
• Vanna R, (Invited Speaker). “Raman and SERS-Based Approaches for the Diagnosis and Monitoring of Cancer and Neurodegenerative Diseases“. RamanFest2016, Berlin, May 19-20 2016.
• Picciolini S “A new approach to characterize exosomes and study their interactions with biomolecules” New Frontiers of Biological Membranes, Milano 30 May 2016.
• Morasso C. “Simultaneous detection of multiple biomarkers by means of SERS on polymer nanopillar gold arrays” Photonic West  BIOS, San Francisco, California. March 7, 2016. Proc. SPIE 9724, Plasmonics in Biology and Medicine XIII, 972404; doi:10.1117/12.2208339
• Morasso C “Simultaneous Detection of Multiple biomarkers by means of  Polymer Nanopillar – Gold Arrays” NanotechItaly, Bologna, Italy 25-27 November 2015.
• Bedoni M. “Studio della nanotossicità a livello cutaneo: metodiche istologiche di indagine” 78° Congresso Nazionale di Medicina del Lavoro, Milano, Italy 25-27 Novembre 2015
• Morasso C. “Ultrasensitive Detection of Multiple Genetic Leukemia Biomarkers by means of Surface Enhanced Raman Spectroscopy” Analitica2015. Trieste, Italy. 13-17 September 2015.
• Vanna R.  “Raman Imaging for the Intracellular Label-Free detection and study of Drug Nanocarriers and Graphene nanoparticles”. Biophotonics2015, Florence, May 20-22, 2015
• Pellacani P, Frangolho A, Lopez Sanchez L, Marabelli F, Morasso C, Manso M, Valsesia A, Marchesini G “Imaging nanoplasmonics (iNPx), a multifunctional technology platform” 4th International Conference on Bio-Sensing Technology. 10-13 May 2015, Lisbon, Portugal
• Morasso C. “Unveiling rehabilitation biomarkers: nanobiophotonics at work” Presented at: Robo-business Europe. Milan, Italy. 29-30 April 2015
• Gualerzi A, Vanna R, Milani A, Niada S, Brini AT, Morasso C, Picciolini S, Bedoni M, Ciceri F, Bernardo ME, Gramatica F. “Label-free characterization of extracellular vesicles from human mesenchymal stem cells by Raman spectroscopy”   Journal of Extracellular Vesicles 2015, 4: 27783 – http://dx.doi.org/10.3402/jev.v4.27783 ISEV 2015 Annual Meeting, Washington DC (USA). 22-26 April 2015
• Picciolini S.  “2D SERS Substrates for Ultrasensitive Detection of Multiple Genetic Leukemia Biomarkers”. Sensors in Medicine 2015. London, 24-26 March
• Bombera R, Pellacani P, Frangolho A, Valsesia A, Marabelli F, Morasso C, Bonnel D, Schafer C, Marchesini G “One chip solution for label free 10^4-5-plex biomolecular interaction monitoring, SERS and MALDI-TOF MS” 2nd International Conference on Label-Free Technologies. 12-14 March 2015, Cambridge, MA, USA
• Vanna R. “Raman microspectroscopy enables the identification of different cell subpopulations in Acute Leukemia patients” III Italian “Meeting on Raman Spectroscopy and Non-linear Optical Effect” (GISR2014). 9-11 June 2014. Parma, Italy
• Bedoni M. “Raman spectroscopy on human skin: perspectives for in vivo diagnosis”. 41th Annual SCUR Meeting. 5-7 June 2014. Monte Porzio Catone, Rome. Italy
• Mehn D. “Bifunctional nanoparticles for surface-enhanced Raman spectroscopy-based leukemia biomarker detection” SPIE Photonics West BIOS 2014, San Francisco 01-07 February 2014. doi: 10.1117/12.2036174
• Vanna R. “Raman spectroscopy for the assessment of acute myeloid leukemia: a proof of concept study” SPIE Photonics West BIOS 2014, San Francisco 01-07 February 2014. doi:10.1117/12.2039186
• Morasso C. “Plasmonic crystal based solid substrate for biomedical application of SERS” SPIE Photonics West BIOS 2014, San Francisco 01-07 February 2014. doi: 10.1117/12.2037780
• Bedoni M. “Un kit diagnotico basato su un biosensore nano strutturato per il monitoraggio della minima malattia residua nella leucemia mieloide acuta” , Nano World Cancer Day 2014, 31 Gennaio 2014. Event organized by the Europen Technology Platform Nanomedicine (ETPN) in 13 countries.
• Morasso C. “Hydroquinone based synthesis of Au nanoparticles with shape control for SERS applications” Italian Crystal Growth 2013, IMEM-CNR, Parma 14-15 November.
• Vanna R. ”Raman spectroscopy for the assesment of acute myeloid leukaemia subtypes”. ECSBM 2013, 25-30 August, Oxford.
• Morasso C. “Star-like gold nanoparticles as highly active substrate for surface enhanced raman spectroscopy “, Progress in Biomedical Optics and Imaging – Proceedings of SPIE, volume 8595, articolo 859507. Colloidal Nanocrystals for Biomedical Applications VIII, San Francisco, California USA, 2-4 Febbraio 2013; doi:10.1117/12.2004046
• Bedoni M. “Microneedles Technology: an Emerging Biomedical Device to Overcome the Skin Barrier”. 39th Annual SCUR Meeting, Lyon, France, 23-25 May 2012. Exp Dermatol (2012) Volume: 21 Issue: 9 Pages: E2-E2
• Mehn D. “Application of gold nanostars in a Surface Enhanced Raman Spectroscopy based method for minimal residual disease monitoring” presented at NanoBioEurope 2012, organized by: European Commission’s Joint Research Centre (JRC) and Fondazione Don Gnocchi, Varese, Italy, 18-20 June 2012.

Master Thesis Works

Domitilla Schiumarini

Title: Sviluppo di un biosensore nanostrutturato per il monitoraggo di biomarcatori genetici della leucemia mieloide acuta
Supervisor: Prof.ssa Paola Giuseppina Sacerdote
University: Universitá Degli Studi di Milano, Facoltá di Scienze e Tecnologie
Year: 2012-2013
Available for consultation after 4 years in the University Library

Silvia Picciolini

Title: Ottimizzazione di nanoholes per il rilevamento di biomarcatori genetici della leucemia mieloide acuta
Supervisor: Prof. Sergio Romeo
University: Universitá Degli Studi di Milano, Facoltá di Scienze del Farmaco
Year: 2012-2013
Available for consultation after 4 years in the University Library

Carla Martino

Title: Sviluppo di un metodo diagnostico per la malattia di Alzheimer basato sull’utilizzo di metodiche spettroscopiche
Supervisor: Dott.ssa Anna Maria Colangelo
University: Universitá Degli Studi di Milano-Bicocca, Corso di laurea magistrale in Biotecnologie Industriali
Year: 2015-2016

Andrea Sguassero

Title: Development of a PCR-free approach based on surface resonance imaging for the multiplexing detection microRNAs related to multiple sclerosis
Supervisor: Prof.ssa Rita Grandori
University: Universitá Degli Studi di Milano-Bicocca, Corso di laurea magistrale in Biotecnologie Industriali
Year: 2016-2017