The Laboratory of Nanomedicine and Clinical Biophotonics (LABION) introduces you another of the five instruments purchase thanks to a grant funded by the Italian Ministry of Health: NanoSigh300 (Malvern Panalytical).
NanoSight300 is a tool that provides an easy-to-use, reproducible platform for nanoparticles characterization.
In fact, this instrument uses the technology of Nanoparticle Tracking Analysis (NTA) to determine the size and concentration of nanoparticles suspended in a liquid. This is possible, because this technology utilizes the properties of both light scattering and Brownian motion (incessant and disordered motion of very small particles suspended in water or gas).
A laser beam passes through the sample chamber and the particles suspended are hit by the laser and scatter the light in such a way that they can be easily viewed thanks to a microscope on which a camera is mounted.
The latter is capable of capturing a video file of the particles moving under the Brownian motion. In addition, the instrument features also a software with an intuitive interface that uses the Stoke-Einstein equation to calculate the hydronynamic diameters of the particles; the measurement is controlled via standard procedures.